Facilities

EQUIPMENT AND INSTRUMENTATION

The Bioelectronics Laboratory lead by Dr. Brian Kim is a part of ECE in College of Engineering and Computer Science as well as Burnett School of Biomedical Sciences (BSBS) in College of Medicine. The lab has five sections: (1) Electrophysiology and Imaging, (2) Prototype Manufacturing, (3) Electronics Design, (4) Biolgical Bench, (5) Tissue Culture. The group has access to many shared facilities, advanced manufacturing facility (AMF), Materials Characterization Facility (MCF), Advanced Research Computing Center (ARCC), and the BSBS-shared facility. Also, the group members have fully access to advanced engineering softwares, inclduing Cadence EDA, COMSOL, ANSYS HFSS, Matlab, and Labview.

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Electrophysiology and Imaging

The fluorescence upright microscope (Zeiss Axio Examiner D.1) is specifically tailored for electrophysiology. It is used for opaque substrates, such as silicon substrate. Reflected microscopy is required to image the cultured cell on the surface. The Axio Examiner D.1 is equipped with reflected bright-field, reflected DIC, and reflected fluores-cence capabilities with high-end objective for water-immersion imaging. Faraday cage offers a low-noise environment for electrophysiology.

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Electrophysiology

The fluorescence upright microscope (Zeiss Axio Examiner D.1) is specifically tailored for electrophysiology. It is used for opaque substrates, such as silicon substrate. Reflected microscopy is required to image the cultured cell on the surface. The Axio Examiner D.1 is equipped with reflected bright-field, reflected DIC, and reflected fluores-cence capabilities with high-end objective for water-immersion imaging. Faraday cage offers a low-noise environment for electrophysiology.

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Patch clamp system

Micromanipulator by Narishige, Zeiss Examiner Upright Microscope

Multichannel Systems (CMOS-MEA5000)

The CMOS-MEA5000-System is the in vitro recording system from Multi Channel Systems for extracellular recordings and stimulation at the highest resolution. Based on the complementary metal-oxide semiconductor technology, it opens up new possibilities in electrophysiological research. With more than 4000 recording sites, each of them sampled at 25 kHz, the chip allows extracellular recordings at a very high spatiotemporal resolution. By including amplification on the chip itself, noise is minimized and a high signal quality is guaranteed.

Recording example from CMOS-MEA5000

High resolution: The video shows the propagation of an average action potential along a single unmyelinated axon of a rat ganglion cell. Details can be found in the paper of H.Stutzki et al. in Frontiers Cell. Neurosci. 8:38 (2014).

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Prototype Manufacturing

The Bioelectronics lab owns fully operational wire bonder (7400A), dual-material 3D printer, laser cutter, fully-automated CNC mill, drill press, laminator, and plasma cleaner for prototyping, early product development, and fabrication process.

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Electronics Design

The lab is equipped with established a CMOS development platform that is capable of designing/simulation/physical layout and post-silicon characterization. Also, we have Metcal soldering station, PCB reflow oven, and heat gun.

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RF Network Analyzer

The network analyzer is for measuring network parameters for high frequencies, between 300 kHz to 3 GHz. This is used to measure inductance and mutual inductance of wireless coils.

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Plasma Cleaner

This is for sample cleaning. Harrick Plasma Inc. 

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Pipette Puller

Sutter Instruments P-87

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Molecular and Cell Biology

Tissue Culture: The group has tissue culture facility and has access to various fluorescence microscopes including upright microscope adequate for imaging cells on opaque substrate (CMOS) and inverted microscopes for typical biological work.

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Bead Beater

 Bead beater is for cell lysis of bacteria and yeast.

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Freeze Dryer

Among the Burnett School of Biomedical Sciences (BSBS) shared facility, we use the freeze dryer to lyopilize biochemical reagents for the portable medical diagnostics project.

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Software

Cadence EDA, COMSOL, Matlab, Labview, and ANSYS HFSS

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Advanced Microfabrication Facility (AMF)

AMF is a multi-user cleanroom facility dedicated to provide university researchers the capabilities to perform cutting edge research, and training and education of students in the use of the available equipment for fabrication and testing of microdevices. AMF consists of a 600 sq. ft. class 100 facility and a 2500 sq. ft. class 1000 facility.
Link: http://www.ampac.ucf.edu/facilities/AMF.php

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Materials Characterization Facility (MCF)

MCF occupies about 7,000 sq. ft. of space and is supported by 3 full-time research engineers and a full-time facilities coordinator. The Materials Characterization Facility (MCF) is dedicated to providing researchers and industrial partners a place to perform characterization and analysis to ad-vance research. We will primarily use SEM, FIB and SIMS to characterize various nanostructures. Link: http://www.ampac.ucf.edu/facilities/MCF.php

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Advanced Research Computing Center (ARCC)

ARCC includes an HPC system (known as Stokes) with over 3,500 cores, 7.5 TB of RAM and 144 TB of storage. Link: https://arcc.ist.ucf.edu/

CONTACT

Email: brian.kim@ucf.edu
Address: HEC-339/BSBS-450

AFFILIATION
  • Department of Electrical and Computer Engineering
  • College of Engineering
  • University of Central Florida

JOINT AFFILIATION
  • Burnett School of Biomedical Sciences
  • College of Medicine
  • University of Central Florida